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A 2025 systematic review of nearly 4 million participants found women on HRT had lower rates of musculoskeletal pain , and Women's Health Initiative data showed estrogen users were more protected from joint replacement and incident joint pain than those on placebo
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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature
Combining multiple compounds that influence similar pathways may increase complexity and variability in outcomes
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