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Cycle Length: Usually run for 4 to 8 weeks, followed by a break of equal length

This supports existing evidence that TNF- is abundantly expressed in adipose tissue and contributes to the chronic low-grade inflammation characteristic of obesity-related insulin resistance 41

All procedures involving experiments on animals have been approved by the HPRA, Ireland and were conducted according to Directive 2010/63/EU of the European Parliament and Council on the protection of animals used for scientific purposes