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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
BPC-157 is studied in laboratory settings for its potential roles in
D50.9 captures the third, the point at which the ida icd 10 code applies once anemia is present
how they translate across routes is an open research question, not a solved one
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