The proteins in the pulldown were resuspended in sample buffer, samples were resolved by NuPAGE gel electrophoresis and anti-MycMunc13-4 (71D10, Cell signaling) or anti-FlagMunc13-4 (Genecopoeia, CGAB-DDK-0050) and GST (Sigma, 06-332) were detected by western blot
BPC-157 is better suited for chronic tendon injuries, gut healing, and situations where you want daily sustained signaling at a lower cost ($250/mo)
The risk profile, the side effect picture, and the lack of medical indication make it a different conversation than the recovery, metabolic, and immune peptides above
Gastrointestinal and Enteric Research As a derivative of a gastric-origin protein, BPC-157 has been studied across gastric ulcer, intestinal permeability, NSAID-induced GI damage, and colitis preclinical models