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The cDNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA), quality was checked using a Bioanalyzer (Agilent, Santa Clara, CA) and then subjected to paired-end (2 150 bp) sequencing using the NovaSeq 6000 (Illumina, San Diego, CA) platform
By reducing excessive inflammation, it supports a balanced immune system and prevents the negative impacts of prolonged inflammatory states on overall health
doi: 10.1016/j.postharvbio.2021.111501 65
Participants were asked not to wear contact lenses two days prior to the study visit, and no fluorescein, anesthetic, dilating or other ophthalmic drops were applied before sample collection
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