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The PCR products were quantified via the Qubit High Sensitivity Assay (Thermo Fisher, Q33231) and TapeStation and were submitted for next-generation sequencing on an Illumina NextSeq platform
And for A1298C, the enzyme activity of heterozygous and homozygous mutant individuals are respectively 83 and 61% of the wild-type subjects 15 , as shown in Fig
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