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Sediment the gel by centrifugation at 500 x g for 5 min and carefully remove the supernatant
The reduced complexity of the DNA sequences after bisulfite conversion (very few Cs and many Ts), together with high-homology regions of the human genome, especially in CG2 and the presence of several genotypes/quasispecies, makes it difficult to have a set of BS-specific primers to successfully amplify the HBV genome of interest in infected tissues
Small 19 , e2303459 (2023)
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