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When combined with protein-complete meals and measured calorie goals, many patients experience consistent fat loss with minimal muscle loss

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The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min

Additionally, we observed an average 74-fold increase in the AP5Z1 GP level at the end of 4th week during 4-week intermittent fasting compared with the level before 4-week intermittent fasting