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A stimulus was considered active when it released the typical head-searching movements (vigorous, low amplitude horizontal and vertical scanning movements of the head displayed after stretching movements towards the rod) usually followed by grasping movements (labial seizing of the rod extremity)

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The norleucine-4 modification eliminates the methionine residue susceptible to oxidation, while the D-phenylalanine-7 substitution enhances receptor binding affinity by constraining the peptide backbone in a conformation that favors MC1R interaction