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The resulting cDNA was used as a template for quantitative RT-PCR, which was performed with primers specific for TGF-1 and -actin (see in Table 1 ), according to the instructions for the SYBR Premix EX Taq Kit (TaKaRa Biotechnology Co., Ltd.), and a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, United States)
Oliero M, Alaoui AA, McCartney C, Santos MM
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Isolation of the thyroid gland from the experimental rats Firstly, the experimental rats were killed by the overdose of ketamine (300360 mg/kg) + xylazine (3040 mg/kg) intraperitoneally and had their thyroids removed
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