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Cycle Guidelines Approach Standard Duration 4-6 weeks Review point Week 4 Best for General research planning Approach Extended Duration 6-8 weeks Review point Week 6 Best for Stubborn-injury research models Approach Long Duration 8-12 weeks Review point Week 8 Best for Slow-remodeling tissue research planning Off periods of 2-4 weeks between cycles are common in research planning

The ranges below are indicative only

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Ferric iron is released with sodium acetate solution (pH 4.8), which is reduced to ferrous iron Fe(II) by sodium ascorbate which then reacts with sodium 3-(2-pyridyl)-5,6-bis(4 phenylsulfonate)-1,2,4-triazine (ferrozine) to form a purple Fe-ferrozine complex that can be read spectrophotometrically (Simpson and Peters 1990)