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In vivo animal experiments showed that daily administration of NNMTi for 28 days to dietinduced obese (DIO) mice significantly upregulated the expression of lipolysisrelated genes (such as ATGL and HSL) and downregulated lipogenesisrelated genes (such as FAS and ACC), indicating that NNMTi effectively promotes fat breakdown and inhibits fat synthesis

By measuring changes in these processes, scientists can study metabolic responses with greater accuracy

Still, that effect did not replicate at scale in the larger, longer Phase IIb study designed to confirm it which is the standard reason a compounds development gets discontinued rather than a sign the compound is unsafe