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3 Roles and response mechanisms of heat shockrelated proteins in the pathogenesis of male infertility 3.1 Composition and functional basis of the HSF/HSP system in dynamic stress responses Under heat stress, the testicular cells activate a series of protective responses, among which heat shock factors (HSFs) and heat shock proteins (HSPs) play central roles (Widlak and Vydra, 2017)

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On the other hand, this action makes 16-a-OHE1detrimentally aggressive in the presence of cancer or certain genetic predispositions to increases in 4-OHE1

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Reaction assay of GPX activity contained 1 mM ethylenediaminetetraacetic acid, 200 mM NADPH, 1 mM NADPH-dependent thioredoxin reductase, 2 mM TRX, and 250 mM H 2 O 2

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