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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

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Potential drug interactions also warrant attention, as probiotics may alter the pharmacokinetics or efficacy of immunosuppressive therapies, thereby increasing infection risk or compromising therapeutic outcomes, posing particular concerns for patients undergoing chemotherapy, organ transplant recipients, and individuals with HIV/AIDS

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