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For transient expression, cells in 12-well plates were typically transfected at roughly 70% confluency using 2.5 l of polyethyleneimine (PEI
Both vials require refrigerated storage at 2-8C after reconstitution a dedicated mini fridge keeps temperatures stable and avoids the door-zone fluctuations of a household refrigerator
Rather than starting at 500mcg, consider beginning at 200-250mcg for the first week
Regular check-ins with your healthcare provider are important to monitor your progress and adjust any other medications you might be taking, such as insulin or other diabetes medications
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