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Insoluble material was removed by centrifugation at 30,000 g for 30 min and the solubilised complex was immobilised by batch binding to M1 anti-FLAG affinity resin in the presence of 5 mM CaCl 2
Products that contain L carnitine are typically taken daily rather than only around workouts, allowing individuals to maintain consistent intake as part of broader nutrition and fitness habits
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Pre-test preparation Fast for 8-12 hours before testing, consuming only water during this period
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