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These steps were followed by incubation with a goat anti-rabbit IgG secondary antibody labeled with Alexa fluor 488 (1:400
10.1016/j.jpba.2013.12.014 4 BortnarR.RepiM.KamerlinS
Map local anisotropic sharpening and real space refinement was carried out with Phenix 92 , while manual model building was done with COOT
The combination protocol is a logical extension of that literature, with mechanistic complementarity supported by the known biology of both compounds
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