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Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

But I think the notion, I think it's the same for example with metformin, even though it gives less weight loss because that has also been tried in both people with diabetes and people without diabetes