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Because it is not practical to measure electron concentrations directly, the electron energy potential of a redox system is determined from the electrical potential or voltage of the individual half cells, relative to a standard half cell

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The following day, samples were washed (5 min each) sequentially in IP buffer, Wash Buffer I (50 mM HEPES/KOH pH 7.5, 500 mM NaCl, 5 mM EDTA pH 8.0, 1% Triton X-100, 0.1% sodium deoxycholate), Wash Buffer II (10 mM Tris-HCl pH 8.0, 250 mM LiCl, 0.5% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8.0), and twice in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA)