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Most people start at 0.25 mg once a week for the first month, then step up gradually to the recommended maintenance dose of 0.5 mg, 1 mg, or 2 mg
Measuring a second messenger (e.g., cAMP or Ca 2+ ) supports pathway engagement, but proving that the pathway is causally responsible for the cellular response requires additional evidence such as receptor antagonists, pathway inhibitors, receptor knockdown or knockout, or orthogonal rescue experiments
Int J Mol Sci 24:1703
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