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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5-CGGTGCCCTGAATGAACTGC-3, reverse primer 5-CAGTCATAGCCGAATAGCCT-3) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)

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Int J Eat Disord 2015;48(7):826-841

And so far, research raises serious concerns