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Mycotoxins (aflatoxin, ochratoxin A, trichothecenes), heavy metals (mercury, lead, arsenic, cadmium), and environmental chemicals all place direct demands on methylation at multiple levels simultaneously
(1) Mechanism of action: Pyridostigmine is a reversible acetylcholinesterase inhibitor that prevents acetylcholine (ACh) breakdown at the neuromuscular junction, thereby increasing neurotransmitter availability and enhancing neuromuscular transmission
Neurological symptoms disrupt hormonal balance while signs of anemia limit oxygen delivery to reproductive tissues
However, basal levels of phase I and most phase II drug metabolizing enzymes in HepG2 cells are very low compared to human hepatocytes (Wilkening et al., 2003
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