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Increased ROS which are produced through the oxidative burst associated with the inflammatory process in the cell may lead to severe damage to macromolecules19

Nevertheless, the aberrant activation of c-Met and downstream signaling pathways through overexpression of HGF or c-Met, gene amplification, mutational activation of c-Met, down-regulation of Met-targeted miRNA, binding to other ligands, autocrine signaling or abnormally high HGF levels initiates and drives tumorigenesis and promotes tumor growth, invasion, metastasis, and angiogenesis in HCC

Validation of biomarkers of aging

Lastly, a genetic knockdown of GPX4 with three different shRNA clones was performed, and p70S6K levels were measured by Western blotting