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The question we keep coming back to is the same one we have always asked

Despite its prevalence, caffeine is addictive, and withdrawal can cause symptoms like headaches, fatigue, and irritability

The sample size calculation was based on an assumed maximum effect size for survodutide vs placebo of a 0.50.6% change (SD 1%) in HbA 1c for the primary endpoint, similar to that seen in a Phase II trial of semaglutide [16]

For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method