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Once the cells reached 80% confluence, which typically occurred within 24 to 48 h, the cells were subjected to a 30-minute period of starvation using freshly prepared Krebs-Ringer-HEPES (KRH) buffer
Shahreyar M, Fahhoum R, Akinseye O, Bhandari S, Dang G, Khouzam RN
The multi-pathway approach aims to mimic the body's natural metabolic regulation more closely
injection of human GLP-1 (3 nmol/100 l saline, CS Bio) or saline at 10:00, then they were administered human ghrelin (1.5 nmol/100 l saline, Peptide Institute) or saline injection at 10:30
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