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Enhanced ATP Assay Kit, Fluo-4 AM, Antifade Mounting Medium with DAPI, and BCA protein detection kit were supplied by Beyotime (Shanghai, China)
2.2 RNA isolation and real-time polymerase chain reaction (PCR) analysis The total RNA was isolated from the heart apex tissue using Tri reagent (Sigma-Aldrich)
It is a selective ghrelin receptor agonist, which makes it useful for isolating GH-axis activity without triggering broad hormonal responses
Subcutaneous administration, where medication is delivered into the fatty tissue layer beneath the skin rather than into muscle, further minimises discomfort
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