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Similarly to OH, periodic acid damages proteins in intestinal villi and induces cell lysis (184)
The cDNA was subjected to RT-PCR with a Hot Start Pol system (Jena Bioscience, Jena, Germany) using GST- specific primers, such as HlGST RT forward and HlGST RT reverse primers, and HlGST2 RT forward and HlGST2 RT reverse primers (Table 1), following the manufacturers instructions
Moreover, free lipoic acid is rapidly eliminated from cells, so any increases in direct radical scavenging activity are unlikely to be sustained
A typical nasal TB-500 protocol might use 500-1000mcg daily during loading phases, dropping to 250-500mcg daily for maintenance
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