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The membrane was incubated with rabbit anti-GPX1 antigen affinity purified monoclonal antibody (Catalog # M01019-1) at 1:500 overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT
Reverse-phase HPLC purity determination Mass spectrometry identity confirmation Copper content verification (ICP-MS or AAS) Appearance and solubility verification Endotoxin screening (where applicable) Related Research Compounds Researchers studying regenerative signaling, extracellular matrix biology, and longevity pathways may also be interested in:
In most of our animal studies, we used a mixture of 2 molecules of GHK to 1 molecule of copper ion to avoid the possible oxidative actions of free copper ion
Surbeck, M., Deschner, T., Schubert, G., Weltring, A
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