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Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA
For example, shifts in estrogen during perimenopause and menopause can affect sleep, recovery, weight, skin, and muscle tone
Skaper SD (2012) The neurotrophin family of neurotrophic factors: An Overview
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