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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C
Standing 10mg prices across this group span $55.00 to $99.99, close to an 80 percent spread for a compound every supplier advertises at similar purity
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BPC-157 is considered safe for use, being naturally found in gastric juices (6)
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