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The sphincter of Oddi was clamped, and 46 ml of cold enzyme buffer (1 Hanks Balanced Salt Solution, 25 mM HEPES, 100 mg l 1 DNase I, 1 penicillinstreptomycin with glutamine)/collagenase (1 mg ml 1 ) was introduced through the bile duct

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Patients on anticoagulants should maintain consistent vitamin K intake and discuss any supplement changes with their healthcare provider