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At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)
In experimental settings, GIP receptor activation by compounds in this class has been studied in the context of: Insulin secretion modulation in pancreatic beta-cell model systems Adipose tissue signaling pathway investigation in preclinical metabolic models Energy expenditure pathway research in rodent experimental subjects GCGR Pathway: What Preclinical Models Suggest
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Laycock-van Spyk S, Thomas N, Cooper DN, Upadhyaya M
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