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For GSEA, genes were ranked by the t-statistic of the Lm coefficient in the linear mixed-effects model and then analyzed for the enrichment of canonical pathways and Gene Ontology term gene sets obtained from MSigDB v2.5
An engineered G s construct (mini-G s ) was used for expression and purification of the compound 2LY3502970GLP-1R complex, which was designed based on that used in the determination of A2AR-mini-G s crystal structure 48
So, the next time you place that vial back into its cool, dark spot in the refrigerator, know that you're not just following a rule
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