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Two 29 to 31-gauge, 0.5-inch insulin syringes (one for adding diluent, one for drawing doses) Alcohol swabs (70% isopropyl alcohol) A clean, hard, flat surface (or a laminar-flow hood if available) A permanent marker and label tape to record the date and concentration on the vial Refrigerator set to 2 to 8 C for post-reconstitution storage Why Not Sterile Water for Injection

However, an optimal gene copy number should be considered because of metabolic burden by protein overexpression ( sod -lc gene expression were constructed under promoter #14 (Figure 3A), which one to four copy number recombinants named as S-3/pKLH167, S-3/pKLH337, S-3/pKLH341, and S-3/pKLH344

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