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93,94 In a randomized placebo-controlled trial that included 48 perimenopausal women, 250 mg fenugreek extract twice daily for 42 days reduced symptoms, especially hot flashes, night sweats, depression, and insomnia, and improved hormone balance by increasing E2, progesterone, and testosterone levels and decreasing levels of FSH and sex hormone binding globulin (SHBG) (a protein that binds estrogen and testosterone, making them unavailable)
As with any medical treatment, there are potential risks, side effects, and contraindications, which will be discussed during a personalized consultation
We still require controlled human trials, standardized dosing, and long-term safety studies
Step 6: Filter if required For cell culture applications, pass the solution through a 0.22 m syringe filter under sterile conditions to remove any particulates and ensure sterility
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