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Protein (50 g) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (412%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

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When acetylcarnitine is injected directly into the brain, most of the acetyl groups were metabolized, as 60% of the injected acetylcarnitine was recovered as exhaled 14 CO 2 after 22 h, and the percentage recovered in the brain was 1.9, 1.6, 1.3, and 0.9% at 1, 3, 6, and 22 h, respectively [109]

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