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The concentration of Human Tie2 in the samples is then determined by comparing the OD of the samples to the standard curve

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Read the absorbance at 405 nm or 415 nm using a microplate reader

We have good news regarding the absorption: on top of manufacturer claims, there is some data (animal study) demonstrating in can get into the skin , and it seems to be better at it than Ascorbyl Glucoside, another vitamin C derivative