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After suspension in complete IEC medium, primary IECs were harvested by centrifugation three times at 100 g for 5 min
Membranes were then blocked in Starting Block T20 solution (Thermo Fisher Scientific) at room temperature for 1 hour, washed 3 times with PBS with Tween-20 (PBST), and incubated with an appropriate dilution of the primary antibody (all antibodies were used at a dilution of 1:1,000 except for GAPDH and -actin, which were used at dilutions of 1:5,000) in Starting Block T20 solution overnight at 4C
Tirzepatide produces more weight loss than semaglutide, but individualized dosing with robust support can achieve similar outcomes using lower doses
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