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Quantification of dimedone signal was performed using flow cytometry and super-resolution microscopy (Structured illumination microscopy, SIM using N-SIM, Nikon)
268,269 Additionally, SREBP activity is reportedly upregulated in tumor-infiltrating Tregs, and blocking SREBP signaling disrupts the activation of PI3K in these cells, further suggesting that lipid signaling enhances the functional specialization of Tregs in tumors
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