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Cells isolation from freshly donated peripheral blood was done using Ficoll-paque plus density gradient centrifugation (Amersham Biosciences, Little Chalfont, UK)

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B12 can also: Improve mood and helps retain memory and mental functions Lower fatigue and reduces muscle weakness Improve metabolism Boost sperm count Prevent or treat diabetic neuropathy, pins and needles (paraesthesia) What symptoms can occur with vitamin B12 deficiency

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