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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18
We identified acetylcarnitine as a potential biomarker for diagnosing depression, determining remission status, and monitoring treatment effectiveness
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