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Synthetic small molecule SIRT1 activators Compared with natural products, synthetic SIRT1 activators offer several advantages, including defined molecular structures, high target selectivity, and stable pharmacokinetic profiles, making them a promising direction for the clinical application of SIRT1-targeted therapies
Weighed cellular pellets were solubilized in ice-cold RIPA buffer (150 mM NaCl, 1% Nonidet NP-40, 1% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) supplemented with a phosphatase inhibitor cocktail (1:200, Sigma-Aldrich P5726) and Benzonase nuclease (1:1000, Merck 70,664)
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